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A Comparison Between Two Assays for the Redox Status in Plasma
Eugene Jansen, Piet Beekhof, Nicole Schupp, Moritz Kreutzmann and Bettina Kraus
In the present study, two commercial assays for the redox status are compared and evaluated, the SH groups in proteins (SHp) and the total thiol levels (TTL). Both assays are colorimetric endpoint reactions and have been adapted for use on an automatic clinical analyzer. Both assays performed well by giving comparable results in plasma samples. A good correlation with a correlation coefficient (R2) of 0.889 was found between the two assays. However, for some pathological specimens, especially highly lipemic samples, the SHp assay performed better than the TTL assay. When plasma samples with a high lipemic index were omitted, the correlation between the two assays was even better with an R2 of 0.942.
In conclusion, both assays for the redox status performed well, but the SHp assay can also be used in high lipemic samples.