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A Rapid and Accurate Microfluidic Detection Method for Amino Acids in Cell Culture Medium

Qinghong Zhang, Liu Tie, Liming Shi

In current biopharmaceutical industry, the development of bioanalytical technology has received more and more attention. The development and optimization of cell culture medium, as one of the key components in biopharmaceutical research and development, largely determines the quality and efficiency of subsequent protein material production. Amino acids are crucial for the cultivation of mammalian cells and the understanding of the concentration of amino acids is important for increasing cell growth and protein productivity in recombinant cell lines. In the process of new drug development, drug proteins are usually expressed by cells or microorganisms. While cells or microorganisms grow and express proteins, nutrients such as amino acids will be continuously consumed. Therefore, different cell culture processes need to monitor the amino acid concentration in the medium to meet the needs of cells for amino acids in time.