毒理学：开放获取

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A Thorough Transcriptome Reveals the Toxicity of Cadmium and A Brand-New Metallothionein in Silkworms

Harry Svenson

Global heavy metal contamination has grown significantly in importance. The fact that heavy metals are absorbed by soil and have an impact on practically all organisms through biological cycles contributes to its broad scope. Through the soil-mulberry-silkworm system, heavy metal poisoning of silkworms (Bombyx mori) prevents larval growth and development and reduces silk production. In the current study, we used transcriptome sequencing of larval midguts exposed to cadmium to investigate the toxicological mechanism of the heavy metal. We discovered that endocytosis, oxidative phosphorylation, and MAPK signalling are three potential pathways that may be involved in cadmium infiltration. Additionally, we discovered a new metallothionein in silkworms that can enhance heavy metal tolerance in B. mori cell lines and is blocked by cadmium exposure. We also created a transgenic silkworm strain that overexpressed metallothionein, and the results demonstrated that this protein significantly improved larval survivability when exposed to cadmium. This study found and functionally validated BmMT, providing a novel possible heavy metal-tolerant silkworm type, and revealed a mechanism for cadmium toxicity.