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Biodegradation and Decolorization of Acid Red by Acinetobacter radioresistens
Ramya Mohandass, Iyappan S, Manju A and Jiffe John S
Acid red decolorizing bacteria was isolated and identified as Acinetobacter radioresistens.The effect of operation parameters such as medium composition, pH, temperature, dye concentration on the decolorization of acid red was studied and the products of degradation were analyzed and confirmed using LC–MS analysis. The reductive cleavage of azo bond was catalyzed by azoreductase, the key enzyme for the azo dye degradation. The catalytic reduction of acid red 37 by purified azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5-[(aminoxy)sulfonyl]phenyl}ethanol and 7,8-diamino-3[(aminoxy)sulfonyl]naphthalene-1-ol.