国际标准期刊号: 2155-9872

分析与生物分析技术杂志

开放获取

我们集团组织了 3000 多个全球系列会议 每年在美国、欧洲和美国举办的活动亚洲得到 1000 多个科学协会的支持 并出版了 700+ 开放获取期刊包含超过50000名知名人士、知名科学家担任编委会成员。

开放获取期刊获得更多读者和引用
700 种期刊 15,000,000 名读者 每份期刊 获得 25,000 多名读者

索引于
  • CAS 来源索引 (CASSI)
  • 哥白尼索引
  • 谷歌学术
  • 夏尔巴·罗密欧
  • 学术期刊数据库
  • 打开 J 门
  • Genamics 期刊搜索
  • 期刊目录
  • 研究圣经
  • 中国知网(CNKI)
  • 乌尔里希的期刊目录
  • 电子期刊图书馆
  • 参考搜索
  • 研究期刊索引目录 (DRJI)
  • 哈姆达大学
  • 亚利桑那州EBSCO
  • OCLC-世界猫
  • 学者指导
  • SWB 在线目录
  • 虚拟生物学图书馆 (vifabio)
  • 普布隆斯
  • 欧洲酒吧
  • ICMJE
分享此页面

抽象的

Characteristic Features of an Analytical Column with a Pentafluorophenylpropyl Stationary Phase Applied To a Determination of a Fluorinated Phenyl Alanyl Derivative Compound, Gw823093, In Human Urine Using an Lc-Esi-Ms/Ms Method

Harue Igarashi, Shizuko Mogi, Akira Wakamatsu, Yoshiyuki Minamide and Shinobu Kudoh

Pentafluorophenylpropyl (PFPP) stationary phases allow for dipole-dipole, pi-pi, charge transfer and ion- exchange interactions and have shown unique retention of small, polar analytes. PFPP columns are able to retain protonated bases in the presence of high percentages of an organic modifier, which is a suitable condition for liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis. Taking advantage of the characteristics of PFPP columns, a liquid chromatography-electrospray ionization coupled with tandem mass spectrometry (LC-ESI-MS/MS) method was developed to quantify the concentration of a fluorinated phenylalanyl derivative compound, GW823093, in human urine. The analyte is retained with great difficulty on an octadecylsilane- bonded silica (ODS) column, but was strongly retained on a PFPP column in 5 mmol/L ammonium formate buffer (pH 2.9) even with an acetonitrile concentration as high as 80%; these conditions protonated the analyte and enabled higher MS signal intensity. However, the analyte peak shape deteriorated after repeated sample injections when urine samples were analyzed under the selected conditions. To solve this problem, an on-line sample clean-up process with an ODS column was employed before the sample was introduced to the PFPP analytical column. A good peak shape without ion suppression was achieved when using acidified buffer containing 10% acetonitrile as a washing solvent for the process, suggesting that components retained by ionic and weakly hydrophobic interactions were removed. This method was optimized and was linear in quantitation in the range of 25-5000 ng/mL, with a mean coefficient of determination over 0.9998. The validated method was successfully applied to a clinical study of GW823093.