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Cloning, Sequencing and Expression of Novel Trichloroethylene Degradation Genes from Stenotrophomonas maltophilia PM102: A Case of Gene Duplication
Piyali Mukherjee and Pranab Roy
A bacterium capable of growth on trichloroethylene as the sole carbon source was identified as Stenotrophomonas maltophilia PM102 by 16S rDNA sequencing (GenBank acc. No. JQ797560). Genomic DNA of this bacterium was amplified using primers specific for the original todC1 gene of Pseudomonas putida F1. Two PCR products were obtained: 300 bp and 350 bp respectively, designated as tce300 and tce350 genes. These novel genes were separately cloned into p-GEMT Easy vector: PR300 and PR350 and sequenced. The sequences have been deposited at NCBI GenBank under accession nos. JX910450 and JX910451.
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