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Cost-Effective Liquid Chromatographic Approach for Rapid Detection of Remdesivir in Pure Form and Pharmaceutical Formulations
Parin Chokshi
The evaluation of Remdesivir in pure form and pharmaceutical dosage formulation has been established and verified using a simple, specific, and repeatable RP-HPLC approach. The estimation was accomplished by Thermo Scientific Hypersil BDS C18 column (150 x 4.6 mm) 5μ i.d. in gradient elution with a mobile phase made up of 10 mM ammonium acetate buffer (pH 3.5 by 2% glacial acetic acid) and acetonitrile, flowing at a rate of 1.0 mL/min. The analyte was measured at a wavelength of 245nm. Remdesivir’s linearity was found to be between 2 and 10 μg/mL, while its recovery was shown to be between 99.01 and 100.04%. The developed method was authenticated as per ICH guideline Q2R2.