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Double-Muscled Phenotype in Mutant Sheep Directed by the CRISPRCas9 System
Mingming Wu
Myostatin (MSTN) is a well-known negative regulator of muscle growth. The double-muscled sheep caused by natural loss-of-function mutations of MSTN have very strong skeletal muscle. In this study, our results demonstrate the successful generation of MSTN mutant sheep via specific targeting of an exon 1 site using Cas9 technology.
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