生物技术与生物材料

抽象的

Effects of Surface Roughness of Hydroxyapatite on Cell Attachment and Proliferation

Ling Li, Kyle Crosby, Monica Sawicki, Leon L. Shaw1 and Yong Wang

Hydroxyapatite (HA) is the main inorganic component of human hard tissue. It is bioactive and supports bone in growth and osteointegration, and thus it is widely used for orthopedic, dental and maxillofacial applications. To fully utilize its potential in forming a strong bone-implant interface, we have investigated the effect of surface roughness of HA on cell attachment and cell proliferation. The HA samples are prepared via sintering, followed by polishing and then scratch generation using a SiC metallographic paper. Cell attachment and proliferation properties are investigated with the aid of ROS 17/2.8 cells and Live/Dead cell staining, while cell morphologies on different surfaces are studied using scanning electron microscopy. It is found that rougher surfaces are better in enhancing cell attachment and proliferation than polished counterparts. It is proposed that the observed phenomenon is related to the enhanced availability of the medium and serum proteins through the grooves underneath the attached cells.