传统医学和临床自然疗法

抽象的

Evaluation of Acaricidal Effect of Ethnoveteinary Medicinal Plant by in vivo and in vitro against Sarcoptes scabiei var. caprae of Infected Goats in North Shoa, Oromia Regional State, Ethiopia

Bedaso Kebede and Tsegaye Negese

This study was conducted to determine parasiticidal efficacy of seven ethnomedicinal plants against Sarcoptes scabiei var. caprae of goats using in vivo and in vitro techniques. In vitro techniques for evaluation of efficacy of medicinal plants essential oils and fixed oils of seven medicinal plant extracts diluted at different concentrations (essential oils from 2.5% to 0.15625% and fixed oils from 160 mg/ml to 5 mg/ml) were added to petridishes containing adult stage of Sarcoptes scabiei var. caprae. After 3 h of contact, all concentrations of essential oil of Eucalyptus globulus and Cymbopogon citractus showed a good in vitro acaricidal efficacy as compared with the non-treated controls (p<0.05). However, Nicotiana tobacum fixed oil had significant (P<0.05) effect at a concentration of 160 mg/ml and 80 mg/ml. Pyrethrum cineraria folium fixed oil showed lower acaricidal efficacy (P<0.05) in all the concentrations of the extract as compared to the reference drugs. In vivo techniques undertaken for evaluation of efficacy essential oil of Eucalyptus globulus and Cymbopogon citractus at a concentration of 0.625% in 2% Tween 80 on two groups of (six animals each) Sarcoptes scabiei var. caprae infested goats were topically treated two times at 14 days interval and its compared with non-treated and treated (diazinone and ivermectin) controls of six goats in each group. The infected goats treated with the essential oils were cured completely. Statistically insignificant (p>0.05) difference was never observed in mite count, Mean Recovery Response (MRR) and degree of Skin Lesion Quality (SLQ) between goats treated with plant extracts and those treated with diazinone and ivermectin. Therefore, Eucalyptus globulus and Cymbopogon citractus extracts should be licensed for the treatment of Sarcoptes scabiei var. caprae.