生物技术与生物材料

抽象的

Genetic Diversity Analysis of Urtica Parviflora in Uttarakhand Himalayas by Rapid Marker

Sandeep Sharma, Syed Atif Ali, Alok Khare and Tapan Kumar Nailwal

Urtica parviflora is herbaceous perennial medicinal plant distributed in Himalayan region from 400m to 3000m amsl altitude range. In the present study, five genotypes of U. parviflora were collected from different altitudes having variability in morphological traits. This genotype was subjected to Random Amplified Polymorphic DNA (RAPD) marker analysis to assess the genetic diversity at molecular level and develop molecular marker for their identification. Fairly rich genetic diversity was found through RAPD marker analysis. A total 66 scorable bands were produced in five samples with 8 primers. Out of 66 bands, 43 bands were found to be polymorphic i.e., 65.15% polymorphism. The dendrogram of samples shows three major clusters. The samples of similar altitudes were found to be present in one cluster.