细胞和分子生物学

抽象的

Genetic screening of Y-STR (DYS389 I/II and DYS385) in a Random Population of Iranian Kurdish Men

Nooshin Hashami Nia, Fatemeh Keshavarzi*, Majid Sadeghizadeh

Introduction: Human Y-chromosome short tandem repeats (Y-STR) are powerful markers in forensic genetics. The objective of this study was to determine the haplotypes and allele frequency of DYS385 and DYS389I/II microsatellites in a random population of Iranian Kurdish men. Materials and methods: Genomic DNA was extracted from 192 Kurdish volunteers using a cinnaclon kit (DN8115c.cn). Genotyping of Y-chromosomal STRs (DYS385 and DYS389I/II) was done by HRM method. Arlequin Software v.3.5 was used to calculate allele number, haplotype frequency, gene diversity (GD) and haplotype diversity (HD). Results: The allelic frequency and number of alleles of DYS385, DYS389I and DYS389II STRs were 0.73, 0.66, 0.37 and 7, 7, 4, respectively. A total of 23 haplotypes were recorded, 8 of which were unique. 9 haplotypes have the greatest frequency in each of the four provinces. In addition, 5 and 3 unique haplotypes were observed in Kurdistan and Kermanshah provinces, respectively. Average locus diversity was 0.887 ranging from 0.01 for DYS385 to 0.659 for DYS389I. Conclusion: The results of N.e (effective number of haplotypes) showed that amongst the four provinces, the Kurdish population of Kurdistan Province had the most effective haplotype. Comparative results demonstrated great similarity to the Kurdish population of Iraq. These loci can be used in forensic medicine since they have high variation and polymorphism.