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Micro Propagation Protocols for Five Sugarcane Cultivars
Gamachu Olani* ,Surafel Shibiru, Abel Debebe
In vitro propagation protocol optimized for five Sugarcane cultivars, namely V2-111, V2-333, V2-444, V2- 555 and V2-999 studied. Stems sterilized with 2% local bleach for 20 minutes within laminar flow before their culture on hormone free basal media for their establishment. MS media with 1mg/L BAP+0.1mg/L GA3 resulted a significant number shoots for cultivars V2-333; and 0.5mg/LBAP+0.5mg/L KN, 0.5mg/LBAP alone and hormone free for V2-999, V2-111 and V2-555 with mean of 10.5, 16.3 and 28.2 shoots respectively. All treatments except hormone free resulted better numbers for V2-444, that is 25.2. Finally, when shoots transferred for their rooting and elongation, 1/2MS supplemented with 3mg/LNAA and 1.5mg/LIBA resulted best numbers of roots across all cultivars with means 5.45 and 5.00 respectively. After 30 days, rooted plantlets acclimatized in maintained greenhouse resulting highest 100% survival for cv V2-555.