生物技术与生物材料

抽象的

Molecular Cloning and In Silico Sequence Analysis of Glycine Betaine Biosynthesis Genes in Bacillus subtilis

Lawrance Anbu Rajan, Krishnakumar Vinodhini, Yeragudipati Rajalakshmi and Vetrivel Umashankar

Glycine betaine (N, N, N-trimethylglycine) is a effective compatible solute, which maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, betaine aldehyde dehydrogenase (GbsA) and betaine alcohol dehydrogenase (GbsB) genes encoding for glycine betaine biosynthesis were PCR amplified from genomic DNA of Bacillus subtilis isolated from salted anchovies (Thrissina thryssa) collected from retail fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that GbsA and GbsB genes contain ORF of 1473 bp and 1182 bp long, encoding 474 and 266 amino acids respectively (GenBank accession nos. FJ823257 and FJ823258). In silico sequence analysis revealed that the GbsA and GbsB sequences of B. subtilis were conserved in many eubacteria.