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Optimisation of the Chitinase Production by Serratia Marcescens DSM 30121T and Biological Control of Locusts
Benine Mohamed Lamine, Bendaha Mohammed Lamine and Abbouni Bouziane
Serratia marcescens DSM 30121T was tested for their chitinolytic activity on colloidal chitin agar. The production of the chitinase by Serratia marcescens DSM 30121Twas optimized by using of different parameters such as temperature, pH-value, colloidal chitin concentration and incubation time. The obtained results showed that the maximum chitinase production (0.556 U/ml) was observed at the pH-value of 6, at 30°C. Similarly, the recorded maximum chitinase production (0.368 U/ml) was obtained in the presence of 2% of colloidal chitin concentration in the culture medium after 144 h incubation time.
Furthermore, Serratia marcescens DSM 30121 T showed clear zones of hydrolysis of colloidal chitin on agar. Therefore, the present study showed that Serratia marcescens DSM 30121 T is good source for chitinase production. Moreover, the strain Serratia marcescens DSM 30121 T can be used for biological control of locusts.