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Muhammad Asif Javaid, Sofia Nosheen, Muhammad Adnan Ayub, Majid Mustafa, Adil Naseer, Amer Iqbal and Waheed Arshad
Biodecolorization of Orange C2RL dye was studied with microbial strains isolated from textile dye waste water. In this work optimization process was also examined by changing different parameters like temperature, pH, concentration of dye and time. Biodecolorization process was monitor and corroborated by using spectroscopic techniques like UVVisible and FTIR spectroscopy and by evaluating Chemical oxygen demand (COD) and total organic carbon (TOC) of the dye. The results evidently showed that the decolorization capability decreased with increasing dye concentration, and a marked prohibition outcome was observed when the Orange C2RL concentrations were more than 100 mg/L. The optimum temperature for bacterial decolorization was 35°C. The maximum decolorization activity occurred at pH 7.5. Analysis of dye UV-vis and FTIR spectra after bacterial treatment demonstrated that the decolorization of dye was due to biodegradation.