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Dr. Uma Shankar MS, Praveen Basappa
The purpose of this overview is to illustrate the history of 2D liquid chromatography, as well as the concepts that underpin it and the ongoing questions it faces. In the qualification and quantification of closely eluting analytes, two-dimensional liquid chromatography is an analytical tool. Many approaches, such as heart cutting technology, have transformed analyte selection in complicated biological mixtures. Multiple heart-cutting, which uses multiple sampling loops, provides multiple peak samples from the first-dimensional analysis without jeopardizing the temporary overlap of second-dimensional analysis.