国际药学与生命科学研究与发展杂志

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EFFECT OF FENOFIBRATE ON FREE RADICALS IN VITRO, LIPID PEROXIDES, ANTIOXIDANT ENZYMES AND LIVER TRANSAMINASES IN HYPERLIPIDEMIC MICE

Librado A. Santiago, Chari Jane G. Rosales, Abegail I. Santillian and Kristin Joyce V. Santos

In this study, fenofibrate was examined both in vitro and in vitro. Its antioxidant activity in vitro was screened using DPPH assay; and specific antioxidant activity was observed using nitric oxide (NO•) assay, hydroxyl radical (•OH) assay and TBARS assay. The same substance was administered in mice to observe its antioxidant enzyme – GSH) and catalase - induction, inhibition of serum transaminases –SGPT and SGOT, and lipid lowering activities. It was observed in vitro that DPPH (IC50 > 0.38 mg/mL), NO• (IC50 = 22.81 μg/mL) and lipid peroxidation (IC50 = 30.37 mg/mL) was inhibited but not •OH (SC50 < 0.05 mg/mL). In vivo experimentation for lipid peroxidation showed that TBARS concentration was decreased by 24.79% while increasing concentrations of both GSH and catalase. Serum transaminases were reduced by 3.65% and 8.93%, respectively. Lipid profiling showed a decline in triglycerides and low density lipoproteins by 54.87% and 16.67%, correspondingly; while the amount of high density lipoproteins was augmented by 45.13%. Fenofibrate scavenges of free radicals, stimulates •OH production that may increase body’s defense against pathogens, boosts liver antioxidant enzyme against free radicals, and lowers serum transaminases. The results strongly suggest that fenofibrate, aside from its lipid-lowering activity may also provide antioxidant defenses.